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[Journal of Clinical Virology] Evaluation of simple nucleic acid extraction methods for the detection of SARS-CoV-2 in nasopharyngeal and saliva specimens during global shortage of extraction kits

Journal of Clinical Virology, 23 June 2020

Allen Wing-Ho Chu, Wan-Mui Chan, Jonathan Daniel Ip, Cyril Chik-Yan Yip, Jasper Fuk-Woo Chan, Kwok-Yung Yuen, Kelvin Kai-Wang To

Highlights:

  • Proteinase K-heat method improves nucleic acid extraction from respiratory specimens.
  • Proteinase K-heat method is most useful during shortage of nucleic acid extraction kits.
  • Proteinase-K-heat method is inexpensive.

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